Premium
Bacteria in manure and on manured and NPK‐fertilised silage crops
Author(s) -
Östling Charlotta E,
Lindgren Sven E
Publication year - 1991
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740550409
Subject(s) - silage , manure , biology , bacteria , crop , bacillus (shape) , spore , clostridia , enterobacter , agronomy , clostridium , food science , botany , microbiology and biotechnology , escherichia coli , biochemistry , genetics , gene
The numbers of bacteria belonging to Enterobacteriaceae (enterobacteria), lactic acid bacteria, Bacillus‐ and Clostridium spores were enumerated in manure and on manured and NPK‐fertilised silage crops. The enterobacteria were biochemically characterised by means of the Minitek system (BBL). More than 90% of the enterobacteria on crops belonged to the genus Enterobacter . A majority of these (72%) were identified as E agglomerans . Manuring did not increase the number of enterobacteria on silage crops. E coli was the most frequent species in manure but was present at 10 −3 times that of the total number of enterobacteria on the crop one week after manuring. The number of Bacillus spores was 20–40 times higher on manured crops and the number did not decline with time, whereas Clostridium spores, coliforms capable of growing at 44°C and E coli were reduced 6, 40 and 20 times respectively between manuring and harvesting (7 weeks). Mechanical harvesting increased the number of Bacillus spores and coliforms capable of growing at 44°C on NPK fertilised crops probably due to soil contamination. Enterobacteria and lactic acid bacteria increased during wilting. The most representative enterobacteria on wilted crops was a specific biovariant, possibly E agglomerans or Rahnella aquatilis .