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Measurement of (1 → 3),(1 → 4)‐β‐ D ‐glucan in barley and oats: A streamlined enzymic procedure
Author(s) -
McCleary Barry V,
Codd Rachel
Publication year - 1991
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740550215
Subject(s) - glucan , chemistry , beta glucan , peroxidase , glucose oxidase , chromatography , reliability (semiconductor) , quantitative analysis (chemistry) , food science , biochemistry , enzyme , power (physics) , physics , quantum mechanics
A commercially available enzymic method for the quantitative measurement of (1 → 3),(1 → 4)‐β‐glucan has been simplified to allow analysis of up to 10 grain samples in 70 min or of 100–200 samples by a single operator in a day. These improvements have been achieved with no loss in accuracy or precision and with an increase in reliability. The glucose oxidase/peroxidase reagent has been significantly improved to ensure colour stability for periods of up to 1 h after development. Some problems experienced with the original method have been addressed and resolved, and further experiments to demonstrate the quantitative nature of the assay have been designed and performed.