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Determination of acrylamide monomer in hydroponically grown tomato fruits by capillary gas chromatography—mass spectrometry
Author(s) -
Castle Laurence,
Campos MariaJesus,
Gilbert John
Publication year - 1991
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740540406
Subject(s) - acrylamide , chemistry , chromatography , monomer , detection limit , solid phase extraction , extraction (chemistry) , capillary electrophoresis , polyacrylamide , gas chromatography , polyacrylamide gel electrophoresis , mass spectrometry , aqueous solution , gas chromatography–mass spectrometry , polymer , biochemistry , organic chemistry , polymer chemistry , enzyme
Tomato ( Lycopersicon esculentum L) fruits from plants grown hydroponically on polyacrylamide gel were obtained in order to assess any possible uptake of acrylamide monomer from the nutrient solution to the fruit during cultivation. Analysis of acrylamide in the gel itself involved aqueous extraction, bromination and then capillary GC determination with nitrogen‐specific detection. By standard addition a level of 0·18 ± 0·01 g kg −1 residual monomer was found to be present in a sample of gel used by an experimental horticultural station. Tomato fruits were analysed by extraction of the aqueous phase, bromination, silica‐gel cartridge clean‐up and capillary GC–MS determination by selected ion monitoring. The recovery of the method was 26–62% but losses throughout were compensated for by use of 2,3‐dibromo‐2‐dimethylpropionamide internal standard. No acrylamide monomer could be detected in tomato fruits from plants grown hydroponically on polyacrylamide gel at a limit of detection of 1 × 10 −6 g kg −1 , demonstrating that the monomer is not transferred from the growing medium into tomato fruits.