Separation and quantitative determination of grape and wine procyanidins by high performance reversed phase liquid chromatography | Zendy

Manuel Jorge | Zendy; Silva Ricardo Da | Zendy; Rosec JeanPhilippe | Zendy; Bourzeix Michel | Zendy; Heredia Nicolas | Zendy

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Separation and quantitative determination of grape and wine procyanidins by high performance reversed phase liquid chromatography

Author(s) -

Manuel Jorge,

Silva Ricardo Da,

Rosec JeanPhilippe,

Bourzeix Michel,

Heredia Nicolas

Publication year - 1990

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740530109

Subject(s) - chromatography , chemistry , elution , wine , high performance liquid chromatography , acetone , extraction (chemistry) , quantitative analysis (chemistry) , acetonitrile , gradient elution , food science , organic chemistry

A method is described for the separation and quantitative analysis of procyanidins in grape and wine. On a polyamide chromatographic column an initial elution with water at p H 7.0 enabled elimination of interfering phenolic acids. A second elution with acetonitrile/water (30:70 v) was performed to extract catechins. A third elution with acetone/water (75:25 v) was carried out to obtain procyanidins. The quantitative determination of procyanidins was done by high performance reversed phase liquid chromatography. The HPLC analysis was brought down to ∼55 min. The method is accurate and reproducible and has an acceptable extraction efficiency.

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