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Separation and quantitative determination of grape and wine procyanidins by high performance reversed phase liquid chromatography
Author(s) -
Manuel Jorge,
Silva Ricardo Da,
Rosec JeanPhilippe,
Bourzeix Michel,
Heredia Nicolas
Publication year - 1990
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740530109
Subject(s) - chromatography , chemistry , elution , wine , high performance liquid chromatography , acetone , extraction (chemistry) , quantitative analysis (chemistry) , acetonitrile , gradient elution , food science , organic chemistry
A method is described for the separation and quantitative analysis of procyanidins in grape and wine. On a polyamide chromatographic column an initial elution with water at p H 7.0 enabled elimination of interfering phenolic acids. A second elution with acetonitrile/water (30:70 v) was performed to extract catechins. A third elution with acetone/water (75:25 v) was carried out to obtain procyanidins. The quantitative determination of procyanidins was done by high performance reversed phase liquid chromatography. The HPLC analysis was brought down to ∼55 min. The method is accurate and reproducible and has an acceptable extraction efficiency.