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Interactions of the intestinal microflora with glucosinolates in rapeseed meal toxicity: First evidence of an intestinal lactobacillus possessing a myrosinase‐like activity in vivo
Author(s) -
NugonBaudon Lionelle,
Rabot Sylvie,
Wal JeanMichel,
Szylit Odette
Publication year - 1990
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740520412
Subject(s) - caecum , meal , rapeseed , in vivo , food science , biology , myrosinase , gut microflora , ileum , lactobacillus , histamine , biochemistry , glucosinolate , bacteria , endocrinology , medicine , fermentation , microbiology and biotechnology , botany , genetics , brassica
Twenty‐three intestinal lactobacillus strains of various origins(human, swine, chicken, rats) were tested. Only one(LEM 220) was found to possess ‘myrosinase‐like’ potentialin vitro and was therefore chosen for further studies in vivo. This work was based on a comparison between rats monoassociated with LEM 220 and fed a diet containing 390 g kg −1 of Darmor OO dehulled rapeseed meal(RM) or a soya bean meal based diet(SM), and germ‐free rats fed the RM diet. The results showed that LEM 220 was responsible for a dramatic goitrogenicity in animals fed the RM diet. No other anatomical modification was seen. Bacterial status or diet did not influence thyroxin plasma levels. Feed intake was identical in all animal groups showing that the decreased alimentary consumption observed with conventional animals was not due to the unpalatability of the diet. The association LEM 220‐RM diet had an increasing effect on histamine concentration in the wall of the upper part of the intestine whereas no major differences were seen in the caecum and the colon.