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Starch determination in horticultural plant material by an enzymic‐colorimetric procedure
Author(s) -
Rasmussen Trevor S,
Henry Robert J
Publication year - 1990
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740520203
Subject(s) - starch , bark (sound) , mangifera , horticulture , chemistry , botany , amylase , shoot , biology , food science , enzyme , ecology , biochemistry
The starch content of horticultural plant material (including leaves and woody tissues such as roots, stems, twigs, trunks and bark) was determined by an enzymic procedure. A heat‐stable α‐amylase and amyloglucosidase were used to hydrolyse the starch in the finely ground plant material to glucose. The glucose produced was measured colorimetrically using a coupled glucose oxidase/peroxidase reaction. Conditions necessary for maximum glucose recovery were established. Plant material analysed included samples from macadamia ( Macadamia integrifolia Maiden and Betche, trunk and bark), mango ( Mangifera indica L; stems and roots), lychee ( Litchi chinensis Sonn; leaves, stems, twigs and roots), pineapple ( Ananas comosus (L) Merrill; leaves, stems and roots) and nectarine ( Prunus persica (L) Batsch var nucipersica (Suckow) C K Schneid; twigs, shoots, branches, limbs, roots and trunk). Starch values ranged from less than 14% to greater than 30%. The method allows assessment of variations in the starch content of perennial plants and may be used in crop management.