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Enzymic hydrolysis of glucosinolates in a low water system
Author(s) -
Hanley A Bryan,
Kwiatkowska C A,
Fenwick G Roger
Publication year - 1990
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740510314
Subject(s) - myrosinase , glucosinolate , hydrolysis , chemistry , pulmonary surfactant , enzymatic hydrolysis , aqueous solution , enzyme , biochemistry , organic chemistry , chromatography , botany , biology , brassica
The hydrolysis of glucosinolates by a co‐occurring thioglucosidase, myrosinase, produces a range of physiologically active compounds including organic isothiocyanates and nitriles. Myrosinase is known to be a membrane‐bound enzyme, thus the reaction with glucosinolates is likely to occur in vivo at an interface. Glucosinolates were hydrolysed in a low water system both with and without surfactant. In the former case, more rapid hydrolysis was observed than occurs in buffered aqueous solution. Desulphoglucosinolates were found to inhibit glucosinolate hydrolysis in this system.