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Acid hydrolysis prior to automatic analysis for starch
Author(s) -
Faithfull Nigel T
Publication year - 1990
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740500314
Subject(s) - hydrolysis , starch , chemistry , acid hydrolysis , chromatography , polysaccharide , extraction (chemistry) , solanum tuberosum , reagent , enzymatic hydrolysis , amylase , cellulosic ethanol , enzyme , biochemistry , organic chemistry , cellulose , biology , botany
The determination of starch in potatoes ( Solanum tuberosum L) by enzymic hydrolysis with amyloglucosidase (Hudson et al 1976), compared with the acid hydrolysis procedure suggested here, has several disadvantages. The extraction and hydrolysis stage is slower (21 h cf 5 h per batch of 30), activity of the enzyme may vary, reagents are more expensive, and complete hydrolysis is more difficult. Its main advantage is that it is more specific, not hydrolysing the non‐cellulosic polysaccharides of the cell walls. This communication examines whether an acid hydrolysis method is an acceptable, even preferable, method for determining starch in freeze dried potato samples. Effects of drying, hydrolysis and associated errors are quantified.