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Method for the determination of indole and skatole in pig fat
Author(s) -
Porter Michael G,
Hawe Samuel M,
Walker Norman
Publication year - 1989
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740490209
Subject(s) - skatole , chromatography , indole test , flame ionization detector , chemistry , gas chromatography , adipose tissue , extraction (chemistry) , solid phase extraction , biochemistry
A quantitative method was developed to determine by gas‐liquid chromatography the concentrations of indole and skatole in pig fat. Methanolic extracts of subcutaneous adipose tissue were de‐fatted by cooling, concentrated using solid‐phase extraction columns, and analysed in a gas chromatograph fitted with a polar capillary column and a flame ionisation detector. The method detects indole and skatole to below 0‐002 mg kg −1 fat.