Premium
Fish species identification by fish muscle dry powder as reference material
Author(s) -
Neti Girija,
Rehbein Hartmut
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740460109
Subject(s) - isoelectric focusing , fish <actinopterygii> , raw material , chromatography , isoelectric point , chemistry , muscle protein , polyacrylamide gel electrophoresis , food science , buffer (optical fiber) , sarcoplasm , biology , biochemistry , fishery , anatomy , endoplasmic reticulum , skeletal muscle , organic chemistry , computer science , enzyme , telecommunications
Abstract A method for preparing dry powders from fish muscles is described. The muscle dry powders (MDP) were extracted with low ionic strength buffer and compared with sarcoplasmic extracts of raw muscles by isoelectric focusing on ultra‐thin layer (100 μm) polyacrylamide gels. The protein patterns of MDP extracts corresponded to the patterns of the acidic proteins of raw muscle extracts. Because of the species specificity of the patterns, MDP can be used as reference material for fish species identification. The acidic proteins remained extractable after storage of MDP for several months at room temperature.