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Sugar and sugar alcohol determination in feedstuffs by HRGC, HPLC and enzymic analysis
Author(s) -
Piccaglia Roberta,
Galletti Guido C
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740450303
Subject(s) - chemistry , chromatography , high performance liquid chromatography , sugar alcohol , sugar , fructose , sorbitol , gas chromatography , sucrose , food science
High resolution gas chromatography (HRGC) and high performance liquid chromatography (HPLC) with refractive index detection (RI) and enzymic analysis (EA) were evaluated for sugar and sugar alcohol determination in pressed beet pulp (P), tomato (T) and apple pomace (A), with maize (M) as a traditional reference forage. The methods were not significantly different for fructose and sucrose, whereas glucose in T and A was overestimated by HPLC as these feedstuffs contain relatively large amounts of mannitol and sorbitol, which co‐elute with glucose in HPLC. HRGC and HPLC capacity factors of several standards are reported, as well as the detection limits of each method. HRGC is about 1000 times more sensitive than HPLC/RI.