Premium
Lipoxygenase from lupin seed: Purification and characterisation
Author(s) -
Olias José M,
Valle Manuel
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740450208
Subject(s) - chromatography , linoleic acid , size exclusion chromatography , fractionation , chemistry , lipoxygenase , enzyme , incubation , ammonium , lupinus , biochemistry , biology , fatty acid , botany , organic chemistry
Lipoxygenase (EC 1.13.11.12) from lupin seeds (Lupinus albus cv multolupa) was partially purified (38.4‐fold) by ammonium sulphate fractionation, hydrophobic chromatography and gel filtration. The enzyme was associated with membrane fragments of high particle weights. Its optimum pH was 6.1 and it had K m values for linoleic and linolenic acids of 0.24 and 0.37 mM, respectively. Molecular weight determination gave a value of 92000. Incubation of linoleic acid with partially purified enzyme afforded a mixture of 13‐hydroperoxy‐9cis, 11‐trans‐octadecadienoic and 9‐hydroperoxy‐10‐trans, 12‐cis‐octadecadienoic acids in which the ratio of isomers was 16:9.