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Use of species‐specific antisera to adrenal heat‐stable antigens for the identification of raw and cooked meats by agar gel diffusion and counter immunoelectrophoretic techniques
Author(s) -
Sherikar Adagonda T,
Khot Jayakumar B,
And Bhushan M Jayarao,
Pillai Shreekumar R
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740440108
Subject(s) - antiserum , agar gel , agar , species identification , biology , food science , precipitin , antigen , immunodiffusion , chemistry , chromatography , microbiology and biotechnology , immunology , zoology , genetics , bacteria
Rabbit antisera to adrenal heat‐stable and ethanol‐precipitable antigens of buffalo, cattle, sheep, goat and pig were used to develop an agar gel precipitation test and a counter immunoelectrophoretic method for the identification of homologous species in raw, partially heated and boiled meat extracts. Immunoabsorption was necessary to make the primary antisera species specific. The specific antisera can be recommended for identification of the species of origin of meats and their mixtures (5‐10% adulteration) in raw, partially heated and cooked states even in the case of closely related species, viz cattle and buffalo, sheep and goat.