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Solid‐phase extraction‐fractionation of alfalfa saponins
Author(s) -
Oleszek W
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740440106
Subject(s) - chromatography , chemistry , fractionation , saponin , extraction (chemistry) , solid phase extraction , sorbent , glycoside , methanol , column chromatography , organic chemistry , adsorption , medicine , alternative medicine , pathology
A simple method was developed to isolate alfalfa saponins and fractionate them into medicagenic acid, hederagenine and soyasapogenol glycosides. This method is based on the selective extraction of saponins from a C 18 solidphase column with methanol‐water solutions. Selectivity of the C 18 sorbent to retain saponins strongly depended on the pH of the loaded sample. This characteristic was the basis of a new method of column chromatography for isolation of individual saponins.