Solid‐phase extraction‐fractionation of alfalfa saponins | Zendy

Oleszek W | Zendy

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Solid‐phase extraction‐fractionation of alfalfa saponins

Author(s) -

Oleszek W

Publication year - 1988

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740440106

Subject(s) - chromatography , chemistry , fractionation , saponin , extraction (chemistry) , solid phase extraction , sorbent , glycoside , methanol , column chromatography , organic chemistry , adsorption , medicine , alternative medicine , pathology

A simple method was developed to isolate alfalfa saponins and fractionate them into medicagenic acid, hederagenine and soyasapogenol glycosides. This method is based on the selective extraction of saponins from a C 18 solidphase column with methanol‐water solutions. Selectivity of the C 18 sorbent to retain saponins strongly depended on the pH of the loaded sample. This characteristic was the basis of a new method of column chromatography for isolation of individual saponins.

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