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Extent of hydrolysis in the intestinal tract and potential absorption of intact glucosinolates in laying hens
Author(s) -
Slominski Bogdan A.,
Campbell Lloyd D.,
Stanger Norman E.
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740420404
Subject(s) - thiocyanate , chemistry , incubation , hydrolysis , gastrointestinal tract , in vitro , zoology , feces , food science , biochemistry , biology , ecology
Balance studies were conducted with intact and caecectomised hens to assess the influence of the caeca on the recovery of intact glucosinolates (IG) in excreta. The hydrolysis of IG in in vitro incubations with gastrointestinal tract contents and with excreta from intact and caecectomised hens was measured to aid in the interpretation of the IG balance data. Decomposition of IG in excreta was shown to result in low recovery of IG in balance trials involving long (22–24 h) collection periods for excreta; however, balance trials with caecectomised hens and in which excreta was collected within 2 h showed high recovery. In vitro incubation studies confirmed the caeca as the major source of the IG hydrolytic activity. Free thiocyanate ion concentration of excreta of intact hens markedly exceeded that of the diet, but hydrolysis of glucosinalbin did not appear to be the major source of the thiocyanate ion.