Premium
An enzyme‐linked immunosorbent assay for diacetoxyscirpenol applied to the analysis of wheat
Author(s) -
Mills E. N. Clare,
Johnston Jane M.,
Kemp Heather A.,
Morgan Michael R. A.
Publication year - 1988
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740420305
Subject(s) - diacetoxyscirpenol , trichothecene , toxin , chromatography , mycotoxin , chemistry , antiserum , enzyme , detection limit , extraction (chemistry) , titer , food science , biology , biochemistry , antibody , immunology
Abstract High‐titre, high‐specificity antisera against diacetoxyscirpenol have been produced in rabbits and used to set up an indirect, microtitration‐plate enzyme‐linked immunosorbent assay for the trichothecene mycotoxin. The assay, with a limit of detection of 2 pg per well, has been applied to the determination of diacetoxyscirpenol in wheat. Cereal extraction consists of a simple solubilisation of toxin in methanol resulting in an assay sensitivity of 300 ng g −1 . Recovery, measured at three different toxin levels, was essentially quantitative.