An enzyme‐linked immunosorbent assay for diacetoxyscirpenol applied to the analysis of wheat | Zendy

Mills E. N. Clare | Zendy; Johnston Jane M. | Zendy; Kemp Heather A. | Zendy; Morgan Michael R. A. | Zendy

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An enzyme‐linked immunosorbent assay for diacetoxyscirpenol applied to the analysis of wheat

Author(s) -

Mills E. N. Clare,

Johnston Jane M.,

Kemp Heather A.,

Morgan Michael R. A.

Publication year - 1988

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740420305

Subject(s) - diacetoxyscirpenol , trichothecene , toxin , chromatography , mycotoxin , chemistry , antiserum , enzyme , detection limit , extraction (chemistry) , titer , food science , biology , biochemistry , antibody , immunology

High‐titre, high‐specificity antisera against diacetoxyscirpenol have been produced in rabbits and used to set up an indirect, microtitration‐plate enzyme‐linked immunosorbent assay for the trichothecene mycotoxin. The assay, with a limit of detection of 2 pg per well, has been applied to the determination of diacetoxyscirpenol in wheat. Cereal extraction consists of a simple solubilisation of toxin in methanol resulting in an assay sensitivity of 300 ng g −1 . Recovery, measured at three different toxin levels, was essentially quantitative.

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