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In vivo assay of nitrate reductase in tea ( Camellia sinensis L.) leaf and root
Author(s) -
Sarmah Tarun Ch.,
Choudhury Mahinath Dev,
Goswami Monoranjan
Publication year - 1987
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740410203
Subject(s) - camellia sinensis , nitrate reductase , shoot , incubation , chemistry , theaceae , botany , horticulture , nitrate , incubation period , fibrous root system , biology , biochemistry , organic chemistry
Assay conditions in vivo for determination of nitrate reductase activity (NRA) in leaf and feeder root tissues of tea bushes ( Camellia sinensis L.) were studied. The composition of the incubation mixture, pH, temperature, period of incubation and tea shoot components were varied. Maximum NRA was obtained in leaf with a medium containing 300 mg Polyclar AT with 300 mg leaf disc in 0.1 M phosphate buffer at pH 7‐5, 0.02 M KNO 3 in a total volume of 5 ml and incubated for 4 hat 30°C in the dark. Propan‐1‐ol inhibited NRA in tea leaf and root. Highest activity was found in the first leaf.