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The ratio of B to C hordeins in barley as estimated by high performance liquid chromatography
Author(s) -
Griffiths D. Wynne
Publication year - 1987
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740380306
Subject(s) - hordein , cultivar , nitrogen , hordeum vulgare , chemistry , chromatography , zoology , agronomy , biology , poaceae , organic chemistry
The ratio of B to C hordeins was determined using high performance liquid chromatography (h.p.l.c.) for a range of spring barley cultivars and the values obtained varied from 1.79 for Kneifel to 4.15 for Goldmarker. Fine grinding of seed samples resulted in a significant increase in the quantity of protein solubilised but relatively little difference was observed in the B and C hordein ratio, which appeared to be independent of protein extraction efficiency. Increased nitrogen content significantly changed the ratio by increasing the proportion of C hordein. A highly significant correlation was found between the B and C hordein ratio and sample nitrogen content. Analysis of single seeds selected at random from within a single sample of Goldmarker revealed considerable variability in the B and C hordein ratios with values differing by over 2 units. Due to the sensitivity of the B to C hordein ratio to various environmental effects, it is unlikely that the ratio will be of great value as an unambiguous aid in the identification of barley cultivars.