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A rapid h.p.l.c. method for the determination of methionine hydroxy analogue free acid in supplemented feeds
Author(s) -
Baudichau Alain,
Bruyer Denis C.,
Ontiveros Renda,
Shermer William D.
Publication year - 1987
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740380103
Subject(s) - chromatography , methionine , extraction (chemistry) , chemistry , hydrolysis , coefficient of variation , monomer , amino acid , biochemistry , organic chemistry , polymer
Two methods are described for determining methionine hydroxy analogue free acid (HMB) in supplemented poultry feeds. The procedure is based on extraction of analogue from the feed, followed by high‐pressure liquid chromatographic measurement of the HMB directly after extraction or, more rigorously, after extraction and hydrolysis of oligomers to monomer. Recoveries of HMB over the supplementation range 0.08–0.25% (by wt) are 98% with coefficients of variation of 5.4% in commercial poultry feeds. A 98% correlation coefficient is obtained when the analyses are compared with the gas chromatographic method.