Enzyme‐linked immunosorbent assay of 3‐acetyldeoxynivalenol applied to rice | Zendy

Kemp Heather A. | Zendy; Mills E. N. Clare | Zendy; Morgan Michael R. A. | Zendy

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Enzyme‐linked immunosorbent assay of 3‐acetyldeoxynivalenol applied to rice

Author(s) -

Kemp Heather A.,

Mills E. N. Clare,

Morgan Michael R. A.

Publication year - 1986

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740370911

Subject(s) - immunoassay , antiserum , detection limit , chromatography , conjugate , chemistry , enzyme , bovine serum albumin , extraction (chemistry) , antibody , microbiology and biotechnology , biochemistry , biology , immunology , mathematics , mathematical analysis

An enzyme‐linked immunosorbent assay for the detection of 3‐acetyldeoxynivalenol has been set up with an antiserum preparation raised against a bovine serum albumin—3‐acetyldeoxynivalenol hemiglutarate conjugate. The assay is an indirect, double antibody immunoassay performed on microtitration plates, and is sensitive (with a limit of detection of 1 pg per well) and specific. The assay has been validated for application to rice with a sensitivity of 1 ng g −1 . Sample extraction is a simple solubilisation procedure. The immunoassay described has a number of advantages over conventional methods of analysis.

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