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Production of antisera against sterigmatocystin hemiacetal and its potential for use in an enzyme‐linked immunosorbent assay for sterigmatocystin in barley
Author(s) -
Morgan Michael R. A.,
Kang Angray S.,
Chan Henry W.S.
Publication year - 1986
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740370909
Subject(s) - sterigmatocystin , hemiacetal , antiserum , chemistry , enzyme , biochemistry , immunogen , chromatography , biology , mycotoxin , stereochemistry , antigen , food science , antibody , immunology , monoclonal antibody , genetics
Highly specific antisera of high titre to sterigmatocystin hemiacetal has been produced in rabbits and used to set up a microtitration plate enzyme‐linked immunosorbent assay. Novel conjugation procedures were used in synthesis of both immunogen and the material used to make the assay solid phase. The assay has been validated for the determination of sterigmatocystin in barley—extraction conditions are such that the toxin is converted quantitatively to the more soluble hemiacetal form.