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A simplified procedure for quantification of potato glycoalkaloids in tuber extracts by H.p.l.c.; comparison with ELISA and a colorimetric method
Author(s) -
Hellenäs KarlErik
Publication year - 1986
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740370810
Subject(s) - glycoalkaloid , chemistry , chromatography , ethanolamine , high performance liquid chromatography , solanum tuberosum , solanaceae , biochemistry , botany , biology , gene
An improved high‐performance liquid chromatography (h.p.l.c.) procedure for determination of glycoalkaloid levels in potato tubers has been developed in which Sep‐Pak cartridges replace the commonly used alkaline precipitation for clean‐up of tuber extracts. Glycoalkaloids are extracted from fresh tuber tissue into an aqueous medium, the extract is submitted to clean‐up and α‐solanine and α‐chaconine are quantitatively separated on a reversed phase column with ethanolamine modifier added to the mobile phase. Potato tubers were comparatively analysed for their glycoalkaloid content by this and two other methods: a recently developed immunoassay (ELISA) and a colorimetric procedure representing the traditional chemical approach. Agreement between the h.p.l.c. and the other methods was good.