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Ochratoxin a in pig kidney determined by enzyme‐linked immunosorbent assay (elisa)
Author(s) -
Morgan Michael R. A.,
McNerney Ruth,
Chan Henry W.S.,
Anderson Peter H.
Publication year - 1986
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740370507
Subject(s) - ochratoxin a , ochratoxin , chemistry , kidney , antiserum , detection limit , chromatography , toxin , enzyme , antibody , food science , microbiology and biotechnology , biology , biochemistry , mycotoxin , immunology , endocrinology
An indirect, double antibody enzyme‐linked immunosorbent assay (ELISA) has been validated for application to pig kidney. The specificity of the anti‐ochratoxin A antiserum employed is such that minimal sample preparation is required prior to assay, with consequent beneficial effects on sample throughput. Kidneys (303) obtained in the UK as being unsuitable for human consumption were examined for ochratoxin A content by ELISA. Of these, 191 (63%) contained no detectable toxin (the detection limit was 0.5 ng g −1 ). Only eight samples (2.6%) contained more than 5 ng g −1 ochratoxin A, and only two were above 10 ng g −1 (at 11.5 and 12.4 ng g −1 ). The significance of these findings is discussed.