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Identification of the species of origin of fresh, cooked and canned meat and meat products using antisera to thermostable muscle antigens by ouchterlony's double diffusion test
Author(s) -
Kang'ethe Erastus K.,
Gathuma Joseph M.,
Lindqvist Kaare J.
Publication year - 1986
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740370210
Subject(s) - antiserum , ouchterlony double immunodiffusion , antigen , biology , species identification , immunodiffusion , wildebeest , oryx , food science , veterinary medicine , immunology , zoology , medicine , ecology , national park
Antisera to thermostable muscle antigens (TMA) from 14 species of bovidae were raised in goats and/or sheep. To achieve species specificity the antisera were absorbed with serum from the other species. While the absorbed antisera to TMA of buffalo, impala, eland, waterbuck, wildebeest and oryx were rendered specific, the antiserum to cattle TMA cross‐reacted with buffalo fresh meat antigens (FMA) and cooked meat antigens (CMA) but not with buffalo thermostable muscle antigens. Fresh and cooked muscle antigens from these two species could be differentiated by the antiserum to buffalo TMA. A similar approach was used to differentiate the FMA, CMA, and TMA of kongoni, topi and wildebeest. Antiserum to cattle TMA proved useful in detecting the presence of beef meat in meat products that had undergone commercial sterilisation.