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Effect of pectic polysaccharidases of Pseudomonas solanacearum on cellular components of some food plants
Author(s) -
Ofuya Christopher O.
Publication year - 1986
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740370207
Subject(s) - pectinase , pectate lyase , enzyme , bacteria , biology , pseudomonas , biochemistry , protoplast , chemistry , genetics
Abstract The biological effects of pectic polysaccharidases of Pseudomonas solanacearum were tested on the tissues of certain food plants. It was found that both the endo‐polygalacturonase (PG) EC.3.2.1.15 and endo‐pectate lyase (PL) EC.4.2.2.2 caused loss of cohesion of the cells, and leakage of K + ions. In addition the enzymes were highly toxic to the cells. Endo‐PL in particular caused ca 82.5% death of potato protoplasts within 1 h of incubation. Data on enzyme absorption showed that tomato tissues (a host plant) absorbed the enzymes whereas there was less absorption of the enzymes by tissues obtained from non‐hosts. These results suggest that the enzymes which are synthesised by the bacterium during its parasitic association with certain food plants affect vital physiological processes of the cells.