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A modified procedure for the purification of vicine and convicine from fababeans ( Vicia faba L.)
Author(s) -
Arbid Mahmoud S. S.,
Marquardt Ronald R.
Publication year - 1985
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740361210
Subject(s) - vicia faba , chemistry , extraction (chemistry) , aqueous solution , ethanol , chromatography , water extraction , glycoside , nuclear chemistry , organic chemistry , botany , biology
A procedure was developed for the simultaneous isolation of pure vicine [2,6‐diamino‐4,5‐dihydroxypyrimidine 5‐(β‐D glucopyranoside)] and convicine [2,4,5‐trihydroxy‐6‐aminopyrimidine 5‐(β‐D glucopyranoside)] from dehulled fababeans. The procedure involved the extraction of the glycosides with an ethanol‐water (70:30, by vol.) solution, concentration of the extract to a minimal volume and adjustment of the pH of the aqueous extract to 7.2. Mixed vicine and convicine crystals, which were formed over a period of from 1 to 4 days, were harvested by centrifuging and washed with water and ethanol. Vicine was readily obtained from the mixed crystals by extraction at a low pH and recrystallised by adjustment of the pH to neutrality. Convicine, which is relatively insoluble in an acidic medium, was solubilised in an alkaline solution and recrystallised at a neutral pH.