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Determination of the glycoalkaloid content of potato tubers by three methods including enzyme‐linked immunosorbent assay
Author(s) -
Morgan Michael R. A.,
Coxon David T.,
Bramham Sara,
Chan Henry WS.,
Van Gelder Win M. J.,
Allison Michael J.
Publication year - 1985
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740360408
Subject(s) - glycoalkaloid , food science , chemistry , enzyme , chromatography , biology , biochemistry , solanaceae , gene
Potato tuber samples of diverse genetic backgrounds were analysed independently for total glycoalkaloid content by three different procedures. Two of the methods used were conventional chemical assays, the third an enzyme‐linked immunosorbent assay (ELISA). Agreement between all three was extremely good, though the ELISA employed much simpler sample preparation, was technically easier to carry out, and could assay more rapidly large numbers of samples. For these and other reasons, the ELISA will find wider application in future.