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Determination of thiobarbituric acid reactive substances (TBARS) in fish tissue by an improved distillation–spectrophotometric method
Author(s) -
Ke P. J.,
Cervantes E.,
RoblesMartinez C.
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740351117
Subject(s) - tbars , thiobarbituric acid , chromatography , chemistry , fish <actinopterygii> , detection limit , distillation , sample preparation , biochemistry , antioxidant , fishery , biology , lipid peroxidation
An improved method for determining the thiobarbituric acid‐reactive substances (TBARS) in fish tissue has been described. The biological sample was digested in acidic media and then separated by using specific reflux distillation. The compounds in distillate were estimated by spectrophotometric measurement at 538 nm after the reaction with 2‐thiobarbutanic acid. The operational errors, interferences and the recovery of malonaldehyde standard and TBARS from tissue sample using the described procedure have been studied. The recommended technique is simple and reproducible with a detection limit of 0.2 nmol TBARS per 10 g of tissue sample and an overall deviation of less than 7%. The recommended method has been applied satisfactorily for evaluation of rancidity studies in various fish samples.