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Determination of volatile carboxylic acids (C 1 –C 5i ) and lactic acid in aqueous acid extracts of silage by high performance liquid chromatography
Author(s) -
Canale Andrea,
Valente Maria E.,
Ciotti Angelo
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740351106
Subject(s) - silage , butyric acid , formic acid , chemistry , lactic acid , isobutyric acid , chromatography , aqueous solution , acetic acid , organic chemistry , food science , bacteria , biology , genetics
A simple isocratic h.p.l.c. technique was developed for the simultaneous quantitative analysis of lactic, formic, acetic, propionic, isobutyric, n ‐butyric and isovaleric acids in aqueous acid extracts of silage. An Aminex HPX‐87H strong cation exchange resin column at 41°C, 0.0025 M H 2 SO 4 mobile phase and ultraviolet detector at 210 nm were utilised. Estimated recoveries of acids added to silage ranged from 98.2 to 103.5%.