Simultaneous determination of proline and betaines by high performance liquid chromatography | Zendy

Ford Clive W. | Zendy

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Simultaneous determination of proline and betaines by high performance liquid chromatography

Author(s) -

Ford Clive W.

Publication year - 1984

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740350811

Subject(s) - trigonelline , chromatography , proline , chemistry , phosphate , elution , cartridge , high performance liquid chromatography , legume , biochemistry , amino acid , botany , biology , materials science , metallurgy

A method is described for the simultaneous determination by reversed‐phase h.p.l.c. of proline, glycinebetaine, trigonelline and stachydrine. The mobile phase was water containing 0.02 M dibutylamine phosphate at pH 3.0. The compounds were separated in a Dextropak Radial‐PAK Cartridge and were detected by a differential refractometer. Total time of elution was 8 min. The method was applied to the analysis of leaves of two tropical grasses and a tropical legume which had been subjected to water stress.

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