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Simultaneous determination of proline and betaines by high performance liquid chromatography
Author(s) -
Ford Clive W.
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740350811
Subject(s) - trigonelline , chromatography , proline , chemistry , phosphate , elution , cartridge , high performance liquid chromatography , legume , biochemistry , amino acid , botany , biology , materials science , metallurgy
A method is described for the simultaneous determination by reversed‐phase h.p.l.c. of proline, glycinebetaine, trigonelline and stachydrine. The mobile phase was water containing 0.02 M dibutylamine phosphate at pH 3.0. The compounds were separated in a Dextropak Radial‐PAK Cartridge and were detected by a differential refractometer. Total time of elution was 8 min. The method was applied to the analysis of leaves of two tropical grasses and a tropical legume which had been subjected to water stress.