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Optimisation of the in‐vivo assay conditions for nitrate reductase in barley ( Hordeum vulgare L. cv. Igri)
Author(s) -
Sym Gordon J.
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740350703
Subject(s) - hordeum vulgare , nitrate reductase , shoot , nitrate , incubation , in vivo , phosphate , chemistry , botany , biology , chromatography , horticulture , poaceae , biochemistry , microbiology and biotechnology , organic chemistry
Assay conditions in vivo for determining nitrate reductase activity (NRA) in shoot and root tissues of winter barley ( Hordeum vulgare L. cv. Igri) were studied. The composition, pH and temperature of the incubation medium, and the size of leaf slice were varied. Maximum leaf NRA was obtained with 5 mm transverse slices incubated at 32 o C in a medium containing 0.1 M phosphate buffer, pH 7.0, 0.02 M KNO 3 , 30 ml litre −1 propan‐1‐ol and 0.1 ml litre −1 Triton X‐100. The optimum assay conditions for roots were similar to those for leaves, except that the pH was 7.5. There was negligible nitrite reductase activity in leaves incubated in the dark.