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Natural plant enzyme inhibitors: A comparative study of the action of legume inhibitors on human and bovine pancreatic proteinases
Author(s) -
Prabhu K. Sudhakar,
Saldanha Kenneth,
Pattabiraman Thillaisthanam N.
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740350311
Subject(s) - trypsin , biochemistry , chymotrypsin , proteases , enzyme , chemistry , proteolytic enzymes , hydrolysis , carboxypeptidase , kunitz sti protease inhibitor , legume , biology , botany
Inhibition of total proteolytic (caseinolytic), tryptic (by hydrolysis of benzoyl arginine p ‐nitroanilide) and chymotrypic (by hydrolysis of acetyl tryosine ethyl ester) activities by ten species of legume seeds on human and bovine pancreatic proteases were compared. Sword bean extract had a negligible action on human enzymes, but could completely inhibit bovine trypsin and chymotrypsin. Indian red wood seed extract was more active on human than on bovine enzymes, whereas soya bean, field bean, kidney bean and bengal gram were more active on the bovine counterparts. While acacia seed extracts displayed more pronounced action on human trypsins and chymotrypsins, it was more effective in inhibiting the total proteolytic activity of the bovine system. Cowpea and red gram were more effective in inhibiting the human chymotrypsins. The relative contributions of trypsins plus chymotrypsins, elastases and carboxypeptidases to the total proteolytic activity of human pancreatic preparation were found to be 50, 25 and 25% whereas in the bovine system the values were 75, 20 and 5%.