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Sorghum cultivar verification by electrophoresis
Author(s) -
Taylor John R. N.,
Schussler Lottelore
Publication year - 1984
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740350102
Subject(s) - prolamin , cultivar , sorghum , glutelin , storage protein , polyacrylamide gel electrophoresis , gel electrophoresis , biology , electrophoresis , tannin , botany , poaceae , chemistry , agronomy , food science , biochemistry , gene , enzyme
After comparing the merits of three different electrophoretic methods of separating cereal prolamins, a procedure for sorghum cultivar verification by urea‐polyacrylamide gel electrophoresis has been developed. The problem of the tannins in high‐tannin (bird‐resistant) cultivars rendering the prolamins insoluble has been overcome by extracting the proteins in the presence of polyvinylpolypyrrolidone. As with other cereals, the electrophoretic pattern of the prolamins was unaffected by the environment in which the plants were grown. Further, the pattern was not affected by long‐term storage of the grain, and cultivars appear to remain unique with respect to their prolamin proteins through many generations.