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Nitrate reductase activity in coconut leaves
Author(s) -
Shivashankar S.,
Ramadasan A.
Publication year - 1983
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740341105
Subject(s) - nitrate reductase , enzyme assay , chemistry , specific activity , nitrate , enzyme , in vivo , yield (engineering) , nitrogen , reductase , biochemistry , food science , botany , horticulture , biology , microbiology and biotechnology , organic chemistry , materials science , metallurgy
The effect of various assay parameters on in‐vivo nitrate reductase (NR) activity in coconut leaf tissue was studied and n ‐propanol was found to inhibit the activity at all levels. The enzyme activity was higher in air than under nitrogen. Addition of NADH to the assay medium did not increase the activity significantly. Light stimulated the activity and the addition of glucose to dark treated leaves did not restore the activity fully. The 14th leaf on the coconut crown is the most appropriate for sampling. The inducible NR activity showed a high positive correlation with annual yield.