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The determination of endogenous urinary nitrogen in protein quality studies with rats
Author(s) -
Eyre Michael D.
Publication year - 1983
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740340910
Subject(s) - excretion , urine , nitrogen balance , endogeny , protein quality , nitrogen , urinary system , dietary protein , endocrinology , medicine , chemistry , zoology , biology , biochemistry , organic chemistry
The determination of the endogenous urinary nitrogen (EUN) excretion in protein quality studies using the Thomas‐Mitchell method with rats has usually been estimated using either a protein‐free or low concentration egg protein diet. Generally no effort has been made to equate EUN excretion with the urinary nitrogen excretion in the urine whilst the rat is in a state of maintenance. An attempt was made to find the diet which would maintain the weight of the rats used to determine the EUN at the time of the balance period of the protein quality test animals. It was found that a diet containing 20 g egg albumen kg −1 came the nearest to fulfilling this criterion and was the diet most suitable for the determination of the EUN.