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Utilisation of Egyptian rice straw in production of cellulases and microbial protein: Effect of various pretreatments on yields of protein and enzyme activity
Author(s) -
ElMasry Hoda G.
Publication year - 1983
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740340709
Subject(s) - cellulase , chemistry , hemicellulose , single cell protein , cellulose , lignin , food science , raw material , trichoderma harzianum , mycelium , fermentation , biochemistry , botany , biology , organic chemistry , biological pest control
Rice straw was chosen as a cheap carbon source for SCP production using a locally isolated fungus identified as Trichoderma harzianum . The chemical constituents of the raw material were cellulose 43%, hemicellulose 26% and relatively low lignin, 16%. The effects of chemical and physical pretreatments on the protein production, cellulase activity and cell mass of T. harzianum have been investigated. Pretreatment of the raw material using a combination of NaOH and high pressure steam treatment increased its microbial digestibility from 24% to 73%. Similar results were obtained by pretreatment with sodium chlorite for holocellulose formation. However, maximum yields of cell mass and protein were obtained by the first method. The organism gave 7.18 g litre −1 cell mass and 2.50 g litre −1 mycelial protein. Treatment with sodium chlorite increased cellulase activities but gave a lower fungal yield. When the NaOH was applied with high pressure steam, the organism produced 32.8% crude protein, a yield similar to that given by the same organism cultured on soluble carboxymethylcellulose.