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The isolation and purification of indole glucosinolates from brassica species
Author(s) -
Truscott Roger J. W.,
Minchinton Ian,
Sang Joseph
Publication year - 1983
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740340308
Subject(s) - indole test , glucosinolate , chromatography , chemistry , sephadex , yield (engineering) , column chromatography , brassica , biology , biochemistry , botany , enzyme , materials science , metallurgy
A method for the purification of indole glucosinolates from cruciferous plants using ion‐exchange chromatography on DEAE‐Sephadex A‐25 is described. The procedure is suitable for the isolation of indole glucosinolates from both seed and leaf tissue. Using this preparative technique a fraction containing intact indole glucosinolates is obtained which can be used for subsequent purification of the individual components. Alternatively, the indole glucosinolates can be desulphated on‐column, and the resulting desulpho‐derivatives purified in high yield by high‐performance liquid chromatography.