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Separation of fructose, glucose and sucrose in fruit by high performance liquid chromatography using UV detection at 190 nm
Author(s) -
Shaw Philip E.,
Wilson Charles W.
Publication year - 1983
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740340116
Subject(s) - sucrose , fructose , chemistry , chromatography , absorbance , ion chromatography , column chromatography , orange (colour) , orange juice , high performance liquid chromatography , food science
Fructose, glucose and sucrose were separated by high performance liquid chromatography (h.p.l.c.) on a 5 μm amine column and detected by absorbance at 190 nm. The procedure quantified these sugars in orange juice and in carambola fruit, but preliminary ion exchange column chromatography was necessary before satisfactory h.p.l.c. results could be obtained. The method is sensitive to 1.2 μg of fructose or 9 μg of glucose or sucrose.