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Problems in predicting the inhibition of human pancreas proteinases by soya bean proteinase inhibitors: In‐vitro assays employing human, bovine and porcine proteinases
Author(s) -
Holm Halvor,
Krogdahl Åshild
Publication year - 1982
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740331115
Subject(s) - enzyme , trypsin , chymotrypsin , biochemistry , in vitro , food science , chemistry , biology
Extracts of seven genetically different soya bean varieties were found to give different inhibition of trypsin and chymotrypsin and total proteolytic activity in human pancreatic juice. Commercially manufactured bovine and porcine enzymes were tested against the same extracts in order to find an assay that could substitute for human enzymes. A total of 11 enzyme and substrate combinations were compared. The effects on caseinolytic activity of human pancreatic juice were considered to be the assay most relevant to human nutrition. Large differences in ranking of the varieties between enzymes and species were apparent, using a constant amount of soya bean extract in the assays. The ranking was found to depend also on the amount of soya bean extract added in the assays. These studies therefore question the relevance to man of results from assays employing bovine and porcine enzymes.