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Studies on dietary fibre. A method for the analysis and chemical characterisation of total dietary fibre
Author(s) -
Theander Olof,
Åman Per
Publication year - 1982
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740330407
Subject(s) - chemistry , lignin , uronic acid , polysaccharide , starch , sugar , chromatography , residue (chemistry) , chloroform , dietary fibre , extraction (chemistry) , organic chemistry , food science
A procedure for the analysis of total dietary fibre (including non‐starchy polysaccharides and Klason lignin) is described. The method consists of extraction of homogenised and/or milled foodstuffs with 80% ethanol and chloroform, and analysis of acidic and neutral polysaccharide constituents, starch and Klason lignin in the residue. Uronic acid constituents were determined by a decarboxylation method, neutral sugar constituents as alditol acetates by a gas‐liquid chromatographic method, starch by an enzymic method, and Klason lignin gravimetrically. The sum of anhydrosugars, anhydrouronic acids and Klason lignin from which the starch content is subtracted provides a measure of the total dietary fibre. The method described is reproducible, fairly rapid and gives informative quantitative characterisation of the fibre constituents.