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Constitution of leguminous seeds. Scanning electron microscopy of proteins prepared from Phaseolus beans
Author(s) -
Alli Inteaz,
Baker Bruce E.
Publication year - 1981
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740321105
Subject(s) - phaseolus , citric acid , sodium hydroxide , isoelectric point , chemistry , scanning electron microscope , extraction (chemistry) , sodium , precipitation , botany , chromatography , nuclear chemistry , food science , biochemistry , biology , materials science , organic chemistry , physics , composite material , meteorology , enzyme
Proteins were isolated from citric acid and sodium hydroxide extracts of navy beans ( Phaseolus vulgaris ), white kidney beans ( Phaseolus vulgaris ) and baby lima beans ( Phaseolus lunatus ). Examination of the proteins under the scanning electron microscope revealed that several types of particulate micro‐structures were present in the proteins obtained by citric acid extraction of the beans followed by precipitation by cooling. Examination of the proteins obtained by extraction with sodium hydroxide solution and isoelectric precipitation revealed the presence of only one type of non‐particulate micro‐structure.