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Methods for the estimation of protein in pigeonpea [ Cajanus cajan (L.) Millsp.] and the relationship between whole grain and dhal protein contents
Author(s) -
Singh Umaid,
Jambunathan Ramamurthi
Publication year - 1981
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740320711
Subject(s) - cajanus , food science , chemistry , biology , botany , agronomy
Protein determinations of 172 grain and dhal (decorticated dry split seed) pigeonpea samples were carried out using three methods: (a) micro‐Kjeldahl (MKJ), (b) colorimetric estimation of NH 4 + with phenol hypochlorite reagents using the Technicon Auto‐Analyser (TAA), and (c) dye‐binding capacity (DBC) method using Acid Orange 12 dye. Protein percentages determined by the TAA and MKJ methods were highly correlated for whole‐grain (0.948**) and dhal (0.967**) samples. The DBC method gave reliable results for dhal samples only. In the DBC procedure, higher protein percentages were recorded with smaller flour particles and longer mixing time, but different temperatures and durations of heating had no effect. Positive and highly significant correlations were obtained between the protein values of whole‐grain and dhal samples in all the methods. Small grains gave a lower correlation between whole‐grain and dhal protein content due to the observed negative correlation between grain size and percentage of seed‐coat.