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Lipolytic activity of fungi on rapeseed oil
Author(s) -
El Azzabi Taher S.,
Clarke James H.,
Hill Simon T.
Publication year - 1981
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740320512
Subject(s) - rapeseed , lipolysis , food science , biology , agar , fungus , aspergillus , chemistry , botany , biochemistry , adipose tissue , bacteria , genetics
Abstract Many species of fungi grow on stored seeds of oilseed rape causing the development of high levels of free fatty acids. However, the presence of a fungus on the seed does not imply that it is lipolytic. The work reported here was performed to test the ability of fungi isolated from stored rapeseed to release fatty acids from refined rapeseed oil. Lipolytic activity was assessed by growing fungi on agar, incorporating the oil as droplets and the indicator Nile blue sulphate. Of 18 species tested, only Aspergillus versicolor showed no lipolytic activity. Another method of testing for lipolysis was used for three of the fungi. They were grown in a liquid medium containing oil and the amount of free fatty acids was measured titrimetrically. The results show that many fungi can cause lipolysis of rapeseed oil, but there are great differences in the lipolytic activity between species.