Premium
Estimation of hexose: Pentose ratios in solution using the phenol‐sulphuric acid method
Author(s) -
Ford Clive W.
Publication year - 1981
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740320210
Subject(s) - hexose , pentose , chemistry , phenol , chromatography , pentose phosphate pathway , adsorption , nuclear chemistry , organic chemistry , biochemistry , metabolism , enzyme , glycolysis , fermentation
A procedure is described whereby the proportion of hexose: pentose in solution may be determined spectrophotometrically. The method uses the phenol‐sulphuric acid reaction and a previously unreported ultraviolet adsorption band at 312‐325 nm in the reacted solutions. The λ max of this band increases with, and is linearly related to, the ratio of hexose to total sugars in the mixture, in the range 10‐80% hexose.