Estimation of hexose: Pentose ratios in solution using the phenol‐sulphuric acid method | Zendy

Ford Clive W. | Zendy

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Estimation of hexose: Pentose ratios in solution using the phenol‐sulphuric acid method

Author(s) -

Ford Clive W.

Publication year - 1981

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740320210

Subject(s) - hexose , pentose , chemistry , phenol , chromatography , pentose phosphate pathway , adsorption , nuclear chemistry , organic chemistry , biochemistry , metabolism , enzyme , glycolysis , fermentation

A procedure is described whereby the proportion of hexose: pentose in solution may be determined spectrophotometrically. The method uses the phenol‐sulphuric acid reaction and a previously unreported ultraviolet adsorption band at 312‐325 nm in the reacted solutions. The λ max of this band increases with, and is linearly related to, the ratio of hexose to total sugars in the mixture, in the range 10‐80% hexose.

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