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An extraction method for evaluating the seed proteins of cowpea [ Vigna unguiculata (L) Walp]
Author(s) -
Tella Ayoni F.,
Ojehomon Ojeaga O.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740311208
Subject(s) - vigna , extraction (chemistry) , globulin , chromatography , chemistry , fraction (chemistry) , polyacrylamide gel electrophoresis , electrophoresis , botany , biology , biochemistry , enzyme , immunology
Simple procedures were tested for the rapid extraction and estimation of albumins, globulins, glutelins and gliadins in cowpea seeds. The extraction methods were based on the differential solubilities of these fractions in buffered 2% NaCl, 0.01 M NaOH and 80% aqueous ethanol. Further analysis of the fractions was carried out by polyacrylamide gel electrophoresis which showed that each fraction was a distinct entity. The total extractable protein contents of the varieties analysed varied from 19.0 to 25.7% but the relative amounts of the protein fractions changed between each other in a complementary way, such that some varieties were rich in a particular protein fraction but deficient in another. The procedure finally adopted appeared to be reasonable and convenient but it was not suitable for very dark‐coloured seeds.