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The determination of myo ‐inositol hexakisphosphate (phytate)
Author(s) -
Cosgrove Dennis J.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740311206
Subject(s) - inositol , phosphoric acid , trichloroacetic acid , chemistry , chromatography , elution , inositol phosphate , ion exchange , phosphorus , extraction (chemistry) , ion chromatography , biochemistry , ion , organic chemistry , receptor
A method for the determination of myo ‐inositol hexakisphosphate (phytate; P 6 ‐ myo ‐inositol) in plant material has been devised which involves the extraction of the sample with 0.3m trichloroacetic acid (TCA), application of the diluted extract to an anion‐exchange resin column in the TCA form, and elution of all phosphoric esters, other than P 6 myo ‐inositol, with 0.1 M TCA. P 6 ‐ myo ‐inositol is then eluted from the column with 1.0M HCl and a suitable aliquot of this solution is assayed for phosphorus.