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An enzymic assay for β‐hydroxybutyric acid in liquid whole egg
Author(s) -
Parry Anne E. J.,
Robinson David S.,
Wedzicha Bronislaw L.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740310907
Subject(s) - chromatography , chemistry , enzyme , biochemistry , dehydrogenase
A rapid biochemical method for the determination of D‐β‐hydroxybutyric acid using a specific enzyme (D‐β‐hydroxybutyric dehydrogenase) is described. The assay is based on the measurement of reduced adenine dinucleotide formed during the enzymic conversion of D‐β‐hydroxybutyric acid to acetoacetic acid. Using the biochemical assay, samples of liquid whole egg obtained from 18‐day‐old incubator reject eggs contained 1.81–2.29 mmol litre‐ 1 D‐β‐hydroxybutyric acid; the limit of reliable detection being estimated as 20 μmol litre‐ 1 for this method.