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Cultivar identification of Vicia faba (L.) by sodium dodecyl sulphate–polyacrylamide gel electrophoresis of seed globulins
Author(s) -
Barratt Daniel H. P.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740310811
Subject(s) - legumin , vicilin , globulin , vicia faba , cultivar , polyacrylamide gel electrophoresis , storage protein , gel electrophoresis , sodium dodecyl sulfate , chemistry , isoelectric focusing , chromatography , biology , botany , biochemistry , enzyme , immunology , gene
The globulin fraction isolation from Vicia faba seeds of different cultivars and different lines of the same cultivar has been examined by sodium dodecyl sulphate‐poly‐acrylamide gel electrophoresis (SDS‐PAGE). The technique separates the globulin polypeptides into three distinct groups, termed A, B and C, with molecular weights above 46 kD, between 30–40 kD and below 26 kD, respectively. Distinct differences between polypeptide patterns of the different cultivars and of the different lines of the same cultivar were observed in groups A and B, little variation occurred in group C. Gradient zonal isoelectric precipitation was used to separate the globulin fraction of different cultivars into vicilin and legumin. Variation in polypeptide composition between cultivars was observed in both vicilin and legumin following SDS‐PAGE. No difference between cultivars was detected in group C polypeptides of legumin.