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Hemicellulosic contamination of acid detergent residues and their replacement by cellulose residues in cell wall analysis
Author(s) -
Morrison Ian M.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740310704
Subject(s) - cellulose , hemicellulose , residue (chemistry) , chemistry , cell wall , sugar , acetic acid , nitric acid , contamination , food science , biochemistry , organic chemistry , biology , ecology
The carbohydrate compositions of acid detergent residues, prepared from 26 plant samples, ranging from fruits, vegetables, legumes, forages and woods, were determined and up to 15.4% of the sugar units present were neutral sugars other than D‐glucose and derived from hemicelluloses. Similar analyses on cellulose residues, prepared by the acetic acid‐nitric acid method, showed up to 9.6% of these residues were derived from hemicellulosic contaminants. These results suggest that care should be taken before using acid detergent residue values to calculate the cellulose and hemicellulose content of plant materials. It is observed that more accurate results will be obtained by using values for cellulose residues and this method is recommended even though it is not absolutely accurate, particularly when analysing plant materials with a high cell wall content.