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The analysis of glucosinolates in Brassica species using gas chromatography. Direct determination of the thiocyanate ion precursors, glucobrassicin and neoglucobrassicin
Author(s) -
Heaney Robert K.,
Fenwick G. Roger
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740310612
Subject(s) - thiocyanate , brassica , chemistry , glucosinolate , indole test , gas chromatography , chromatography , ion chromatography , botany , organic chemistry , biology
A method using gas chromatography (g.c.) is described for the analysis of intact glucosinolates in Brassica leaf and seed material. Both glucobrassicin and neoglucobrassicin (indole glucosinolates) are separated using this system and summation of the contents of these indole compounds agrees well with the figure arrived at by a conventional (thiocyanate release) method. The other main thiocyanate ion precursor, glucosinalbin, is well separated from glucobrassicin and neoglucobrassicin and all three may be separated and quantified. The g.c. method has considerable advantages over the alternative method.